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. 2017 Mar 1;8:86. doi: 10.3389/fphys.2017.00086

Table 1.

Information of primers.

Genes Tm (°C) Sequences (5′ → 3′)
mPDE4A 55 (F) TTC AAG CTG CTG CAA GAA GA
(R) TTC CTG AGG ACC TGG ATA CG
mPDE4B 55 (F) GAA CAA ATG GGG CCT TAA CA
(R) TTG TCC AGG AGG AGA ACA CC
mPDE4C 57 (F) CAT GCT CAA CCG TGA GTT GT
(R) TGG AAC GTC TTG AGG AGG TC
mPDE4D 57 (F) GGA GCT TGT CAC CTT CTT GG
(R) GTG GGC TTT AAG TTG CTC CA
mGAPDH 55 (F) TTA GCC CCC CTG GCC AAG G
(R) CTT ACT CCT TGG AGG CCA TG
mIL-6 63 (F) GAG GAT ACC ACT CCC AAC AGA CC
(R) CTA TGG TAC TCC AGA AGA CCA GAG
mNOX2 57 (F) GTG TTG CTC GAC AAG GAT TC
(R) CTC CGA ATG GTT TTG GTA GAG
mNOX4 58 (F) CAG CTT CTA CCT ACG CAA TAA G
(R) GGA AAT GAG CTT GGA ACT TGG
hPDE4 62 (F) GTT GAG ACG AAG AAG GTG ACC AG
(R) GTC GGC CCA TGT TTC CCA CAA TG
hGAPDH 52 (F) GTC GGA GTC AAC GGA TT
(R) GCC ATG GGT GGA ATC ATA