Figure 1.

(A) Quantification of collagen deposition in the lung of intratracheally instilled with a single (bleomycin once) or double (bleomycin twice) dose of bleomycin and compared with single (saline once) or double (saline twice) dose vehicle (saline) treated mice at 7, 14, and 21 days posttreatment. (B) Ashcroft frequency distribution of single (bleomycin once) or double (bleomycin twice) dose of bleomycin and compared with double dose vehicle (saline) treated mice at 7, 14, and 21 days posttreatment. The total Ashcroft score has been divided in three classes (1–3, mild; 4, moderate; ≥5, severe fibrosis). (C) Representative images of mouse lung sections treated and untreated with bleomycin and used for the above of the quantification (10×; scale bar is equal to 100 μm). Nine animals were used for each time point, and each time point was repeated three times. The data represent the mean ± SEM of a total of 27 animals. Changes were compared to the vehicle groups using ANOVA followed by Dunnett’s test. *p < 0.05; **p < 0.01 and ^#p < 0.05; ^##p < 0.01 when changes were compared to each group vs every other group using ANOVA followed by Tukey’s multiple comparison test.