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. 2017 Mar 1;8:199. doi: 10.3389/fimmu.2017.00199

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Copyright © 2017 Stellari, Ruscitti, Pompilio, Ravanetti, Tebaldi, Macchi, Verna, Villetti and Donofrio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Cytokines/chemokines (A) G-CSF; (B) RANTES; (C) IL 12p40; (D) KC; (E) IL 10, determination into the bronchoalveolar lavage fluid of mice lung intratracheally instilled with a single (bleomycin once) or double (bleomycin twice) dose of bleomycin and compared with single (bleomycin once) or double (bleomycin twice) dose of vehicle (saline) treated control groups. Determination was made at 7, 14, and 21 days posttreatment. Nine animals were used for each time point, and each time point was repeated three times. The data represent the mean ± SEM of a total of 27 animals. Changes were compared to the vehicle groups using ANOVA followed by Dunnett’s test. *p < 0.05; **p < 0.01 and ^#p < 0.05; ^##p < 0.01 when changes were compared to each group vs every other group using ANOVA followed by Tukey’s multiple comparison test.