Table 1.
Strains and plasmids used in this study.
| Strains | Description | Source or reference |
|---|---|---|
| Riemerella anatipestifer ATCC 11845 | Serotype 6 | ATCC |
| R. anatipestifer CH-2 | Serotype 2 | Laboratory collection |
| RA-CH2Δ1769 | RA-CH2Δ1769, SpcR | This study |
| RA-CH2Δ1772 | RA-CH2Δ1772, CfxR | This study |
| RA-CH2Δ1769Δ1772 | RA-CH2Δ1769Δ1772, SpcR CfxR | This study |
| RA-ATCC11845 (pLMF02) | R. anatipestifer ATCC11845 carrying pLMF02, AmpR CfxR | This study |
| RA-ATCC11845 (pLMF02:: cat) | R. anatipestifer ATCC11845 carrying pLMF02:: 1769, AmpR CfxR | This study |
| RA-ATCC11845 (pLMF02:: catH79A) | R. anatipestifer ATCC11845 carrying pLMF02:: H79A, AmpR CfxR | This study |
| Escherichia coli strains | Description | Source or reference |
| X7232 | endA1 hsdR17 () glnV44 thi-1 recA1 gyrA relA1Δ(lacZYA-argF)U169λpir deoR (Φ80dlac Δ(lacZ)M15) | Roland et al., 1999 |
| X7232 (pRE112:: 1769USD) | E. coli X7232 pRE112:: 1769USD, SpcR CmR | This study |
| X7232 (pRE112:: 1772UCD) | E. coli X7232 pRE112:: 1772UCD,CfxR CmR | This study |
| X7213 | thi-1 thr-1 leuB6 glnV44 fhuA21 lacY1 recA1 RP4-2-Tc::Mu λpirΔasdA4Δzhf-2:: Tn10 | Roland et al., 1999 |
| X7213 (pRE112:: 1769USD) | E. coli X7213 pRE112::1769USD, DAP, SpcR CmR | This study |
| X7213 (pRE112:: 1772UCD) | E. coli X7213pRE112:: 1772UCD, DAP CfxR CmR | This study |
| BL21(DE3) | E. coli BL21(DE3), expressing host cell | Laboratory collection |
| BL21(DE3) (pET30a) | E. coli BL21(DE3) carrying pET30a, KanR | This study |
| BL21(DE3) (pET30a:: cat-s) | E. coli BL21(DE3) carrying pET30a:: cat-s, KanR | This study |
| BL21(DE3) (pET30a:: catH79A-s) | E. coli BL21(DE3) carrying pET30a:: catH79A-s, KanR | This study |
| S17-1 | Thi-1 thr leu tonA lac Y supE recA::RP4-2-Tc::Mu KanR | Miller and Mekalanos, 1988 |
| S17-1 (pLMF02) | S17-1 carrying pLMF02, AmpR CfxR | This study |
| S17-1 (pLMF02:: cat) | S17-1 carrying pLMF02:: cat, AmpR CfxR | This study |
| S17-1 (pLMF02:: catH79A) | S17-1 carrying pLMF02:: catH79A, AmpR CfxR | This study |
| Plasmids | Description | Source or reference |
| pET30a | pBR322 lacZ, IPTG-inducible promoter, KanR | Laboratory collection |
| pET30a:: cat-s | pET30a carrying cat adding his tag from R. anatipestifer CH-2, KanR | This study |
| pET30a:: catH79A-s | pET30a carrying catH79A adding his tag from R. anatipestifer CH-2, KanR | This study |
| pLMF02 | shuttle vector transferred between E. coli and R. anatipestifer AmpR, KanR | Liu et al., 2016 |
| pLMF02:: cat | pLMF02 carrying cat from R. anatipestifer CH-2, AmpR CfxR | This study |
| pLMF02:: catH79A | pLMF02 carrying catH79A, AmpR CfxR | This study |
| pYES1new | YAC-BAC shuttle plasmid with SpcR | Laboratory collection |
| pRE112 | sacB mobRP4 R6K ori CmR,pRE112-T-vector | Laboratory collection |
| pRE112:: 1769USD | pRE112 carrying 1769USD from R. anatipestifer CH-2and plasmid pYES1new, SpcR CmR | This study |
| pRE112:: 1772UCD | pRE112 carrying 1772UCD from R. anatipestifer CH-2 and plasmid pCP29, CfxR CmR | This study |
ATCC: American Type Culture Collection.