Figure EV1. CPSF3 localizes in the nucleus of Toxoplasma gondii .

1. Schematic view of the cleavage and polyadenylation specificity complex that, in some eukaryotes, is responsible for processing the newly synthesized pre‐mRNAs. CPSF‐73, in humans, has the endonuclease activity and acts concertedly with other CPSF subunits, polyadenosine polymerase (PAP) and other factors. Please note that the number of CPSF subunits is dependent on each species and this model is just shown for reference.
2. A homologous recombination‐proficient T. gondii strain (RH ku80) was used for endogenous epitope tagging of T. gondii CPSF3‐HAFLAG (red) hosted in HFF. Staining of CPSF3‐HAFLAG was done with anti‐HA antibodies and DNA nuclei with Hoechst (blue).