Chromatograms of the sequences of wild‐type
TgCPSF3 and mutants Y328C (A) or Y483N (B). Parasites were transfected with a CRISPR/Cas9 vector producing a single guide RNA (sgY328C, sgY483N or sgE545K) to target the Cas9 editing enzyme to 20‐bp sites on wild‐type
CPSF3 (see Fig
2). After cleavage by Cas9, homology‐dependent repair from a 120‐base donor oligonucleotide (Y328C, Y483N or E545K) resulted in incorporation of the specific SNP (shown in red). For clarity, only chromatograms of Y328 and Y483N are shown. E545K data is shown in Fig
2B.
