Figure EV1. Re‐expression of the AXIN1 coding sequence re‐sensitizes VACO6_R cells to PORCN inhibition.

1. qRT–PCR evaluation of PTPRK‐RSPO3 fusion transcript expression in VACO6, VACO6_R, and HCT116 cells, as indicated. Data are expressed as means ± SD of three technical replicates from one representative experiment.
2. Western blot showing AXIN1 protein expression in VACO6_R, VACO6, and VACO6_RAXIN1FL cells, as indicated.
3. Flow cytometry analysis of the GFP signal in VACO6, VACO6_R, and VACO6_RAXIN1FL cells, all transduced with the 7xTcf‐eGFP WNT reporter.
4. ATP‐based assay measuring viability, after 6 days of LGK974 treatment, of parental VACO6, VACO6_R, and VACO6RAXIN1FL cells, as indicated. Data are expressed as average ± SD of six technical replicates.

Source data are available online for this figure.