Figure EV2. Activated β‐catenin transduction or transient APC downregulation in VACO6 cells induce increased nuclear β‐catenin levels.

1. qRT–PCR evaluation of APC mRNA expression in VACO6 cells transfected with control siRNA or APC siRNA, as indicated. Two‐sided Student's t‐test, ***P‐value < 0.001, P = 8.24E‐04. Data are expressed as means ± RMSE of three technical replicates from one representative experiment.
2. Representative confocal microscopy images of VACO6 cells (as indicated) showing β‐catenin distribution and GFP expression. Nuclei are stained with DAPI (blue). Scale bars, 10 μm.