Figure 3. Cortical infection threads and peg infection in Gifu and epr3 mutants.

Images of cortical infection threads in nodules of Gifu inoculated with R7A DsRed (a–d), epr3-9 mutant inoculated with R7A DsRed (e–h), epr3-11 inoculated with R7A DsRed (i–l) and Gifu inoculated with R7AexoB DsRed (m–p). At 10–14 dpi, nodules were sectioned by hand and cell walls, and infection threads were stained with 0.04% calcofluor. White arrows indicate transcellular infection threads. Red arrows indicate peg-type intercellular infection. Yellow arrows indicate infection thread protrusions. Scale bars, 10 μm.