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RT–qPCR analysis of VSP2 expression in C. rubella seedlings after treatment with water (mock) or 100 μM MeJA for the indicated time periods. Bars represent means and standard errors of the log2 expression levels relative to Actin2 calculated from two independent experiments using a mixed linear model. Asterisks indicate statistically significant differences compared to the mock controls at each time point (***P < 0.001; two‐tailed t‐tests).
