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. 2017 Jan 27;9(3):353–370. doi: 10.15252/emmm.201606547

Figure 1. Decreased protein translation in KO cells.

Figure 1

Surface sensing of translation (SUnSET) analysis was used to evaluate the incorporation of puromycin into nascent polypeptides.
  1. Representative image of Western blot analysis of WT and KO cells treated with puromycin (1 μM) for 30 min. Western blot with anti‐vinculin antibody and Ponceau S staining were used as loading controls.
  2. Total intensity of puromycin‐labeled polypeptides was quantified. Student's t‐test was used for statistical analysis. Data are mean ± SE. ***P < 0.001 (P = 0.0009; n = 4).
Source data are available online for this figure.