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. 2017 Jan 27;9(3):353–370. doi: 10.15252/emmm.201606547

Figure EV5. Effect of l‐arginine on 4E‐BP1 and eIF2α in KO cells.

Figure EV5

Immunoblot analysis showing the effect of arginine treatment (5 mM) in KO myotubes. NT, not treated. Arginine was added to KO cells grown in differentiation medium on days 4, 5, 6, and 7; 15 min after last arginine addition (day 7), cells were lysed and immunoprobed with the indicated antibodies. Vinculin was used as a loading control. Of note, arginine treatment (5 mM) reduced the level of total 4E‐BP1 and decreased the levels of p‐eIF2α. A decrease in the amount of total 4E‐BP1 without much change in the p‐4E‐BP1 leads to an increase in the p‐EBP1T37/46/total ratio, indicating mTOR activation. Data are mean ± SE; Student's t‐test; *P < 0.05; n = 3.Source data are available online for this figure.