Figure 4.

Effect of RAD001 on cell cycle-related changes in VSMCs stimulated with PDGF-BB. (a) pXp27; (b) PDGF-BB+pXp27; (c) PDGFBB+ pXp27+RAD001. Fractions of the cell population in the G0/G1 and S/G2/M cell cycle phases were evaluated and expressed in percentages (%). N=6 in each group. **P< 0.01 vs. pXp27, ##P<0.01 vs. PDGF-BB+pXp27. pXp27: VSMCs were transfected with 5-μg pXp1 reporter plasmid with pXp27 promoter activity; PDGF-BB+pXp27: pXp27 group cultured with 10 ng/mL PDGF-BB for additional 24 h; PDGF-BB+pXp27+RAD001: pXp27 group cultured with 10 ng/mL PDGF-BB and 10 nM RAD001for additional 24 h