Table 2.
Method validation parameters for pig urine.
| Analyte | RA a ± RSD c (%) | LOD b in Solution (ng/mL) | LOQ b in Solution (ng/mL) |
|---|---|---|---|
| α-ZEL d | 89 ± 8 | 0.11 | 0.38 |
| β-ZEL e | 84 ± 9 | 0.16 | 0.54 |
| ZEN f | 95 ± 9 | 0.15 | 0.49 |
| ZEN-14-Glc g | 88 ± 9 | 0.07 | 0.23 |
| ZEN-16-Glc h | 96 ± 9 | 0.18 | 0.60 |
| ZEN-14-S i | 98 ± 10 | 0.02 | 0.08 |
| α-ZEL-GlcA j | 113 ± 6 | 0.73 | 2.4 |
| β-ZEL-GlcA k | 110 ± 7 | 0.42 | 1.4 |
| ZEN-14-GlcA l | 96 ± 9 | 0.30 | 1.0 |
a Apparent recovery; b Limits of detection and quantification in spiked diluted blank urine; c Relative standard deviation; d α-zearalenol; e β-zearalenol; f zearalenone; g zearalenone-14-O-β-glucoside; h zearalenone-16-O-β-glucoside; i zearalenone-14-sulfate; j α-zearalenol-14-glucuronide; k β-zearalenol-14-glucuronide; l zearalenone-14-glucuronide.