Table 1.
Experimental design used in the response surface methodology studies based on three independent variables and the observed responses of F. graminearum strain 29.
| No. | X1 | X2 | X3 | Mycotoxin Levles (ng/mL) | |
|---|---|---|---|---|---|
| PH | Temperature (°C) | Time (day) | DON | ZEA | |
| 1 | −1 (3) | −1 (15) | 0 (21) | 961.02 ± 35.78 | 419.97 ± 30.25 |
| 2 | 1 (9) | −1 (15) | 0 (21) | <LOD | 19084.20 ± 1102.56 |
| 3 | −1 (3) | 1 (25) | 0 (21) | 1493.05 ± 91.23 | 609.66 ± 41.35 |
| 4 | 1 (9) | 1 (25) | 0 (21) | <LOD | 13457.90 ± 978.25 |
| 5 | −1 (3) | 0 (20) | −1 (14) | 585.59 ± 39.75 | 818.34 ± 54.25 |
| 6 | 1 (9) | 0 (20) | −1 (14) | <LOD | 13941.4 ± 886.14 |
| 7 | −1 (3) | 0 (20) | 1 (28) | 2003.54 ± 123.47 | 2288.42 ± 231.52 |
| 8 | 1 (9) | 0 (20) | 1 (28) | <LOD | 20047.30 ± 1203.59 |
| 9 | 0 (6) | −1 (15) | −1 (14) | 1559.09 ± 101.27 | 4118.31 ± 334.59 |
| 10 | 0 (6) | 1 (25) | −1 (14) | 1405.5 ± 89.58 | 2259.09 ± 189.67 |
| 11 | 0 (6) | −1 (15) | 1 (28) | 2098.03 ± 128.71 | 8661.47 ± 678.15 |
| 12 | 0 (6) | 1 (25) | 1 (28) | 2605.26 ± 134.55 | 5319.75 ± 408.14 |
| 13 | 0 (6) | 0 (20) | 0 (21) | 2276.33 ± 121.30 | 3198.34 ± 278.27 |
| 14 | 0 (6) | 0 (20) | 0 (21) | 2313.33 ± 108.24 | 3133.31 ± 256.77 |
| 15 | 0 (6) | 0 (20) | 0 (21) | 2332.08 ± 107.57 | 3126.45 ± 281.73 |
| 16 | 0 (6) | 0 (20) | 0 (21) | 2283.66 ± 119.35 | 3156.87 ± 312.25 |
| 17 | 0 (6) | 0 (20) | 0 (21) | 2376.34 ± 123.83 | 3185.81 ± 243.51 |