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. 2017 Feb 18;9(2):68. doi: 10.3390/toxins9020068

Figure 4.

Figure 4

Effects of bvPLA2 on AD-like skin lesions of CD25 depleted mice. The female BALB/C mice received once with a dose of 0.25mg/kg of rat anti-mouse CD25 (PC61) or total rat IgG on days 3, 4, 11, 17, and 25. (A) CD4+CD25+ populations were measured using FACS on day 1 and 7; (B) The ear thickness was measured using a dial thickness gauge 24 h after the DFE/DNCB application. The concentration of (C) IL-4; (D) IL-13; (E) IFN-γ; (F) TNF-α; and (G) IL-6 were measured by mouse CBA (Th1, 2, and 17) kit and ELSA kit. NC: normal control group; AD: DFE/DNCB applied group; bvPLA2: DFE/DNC applied and bvPLA2 (80 ng/Ear)-treated group. IgG: non-CD25 depleted mice; PC61: anti-CD25-depleted mice. The statistical analyses were conducted with a one-way ANOVA followed by Newman-Keuls multiple comparisons test (** p < 0.01 and * p < 0.05, ## p < 0.01 and # p < 0.05; not significant = ns, n = 3).