Table 2.
Strains and plasmids used in this study
| Strains/plasmids | Genetic characteristics | Source |
|---|---|---|
| Strains | ||
| MG1655 | Wild type E. coli K-12 strain | Lab collection |
| ΔompF | MG1655, ΔompF | This study |
| ΔfadD | MG1655, ΔfadD | This study |
| ΔfadL | MG1655, ΔfadL | This study |
| Pla-empty | MG1655, pACYC184-Kan | This study |
| Pla-fadL | MG1655, pACYC184-Kan-fadL | This study |
| Gen-empty | MG1655, ldhA::FRT-cat-FRT | This study |
| Gen-fadL | MG1655, ldhA::FRT-cat-FRT, fadL | This study |
| M1-12-fadL | MG1655, FRT-cat-FRT, M1-12-fadL | This study |
| M1-37-fadL | MG1655, FRT-cat-FRT, M1-37-fadL | This study |
| M1-46-fadL | MG1655, FRT-cat-FRT, M1-46-fadL | This study |
| M1-93-fadL | MG1655, FRT-cat-FRT, M1-93-fadL | This study |
| ΔompF + Pla-empty | MG1655, ΔompF, pACYC184-Kan | This study |
| ΔompF + Pla-fadL | MG1655, ΔompF, pACYC184-Kan-fadL | This study |
| Plasmids | ||
| pACYC184-Kan | p15A, pACYC184, Kanr | This study |
| pACYC184-Kan-fadL | pACYC184-Kan harboring fadL, Kanr | This study |
| pXZ18Z (TE) | pTrc99a-Ricinus communis thioesterase-fabZ, Ampr | [42] |