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. 2017 Feb 28;17:23. doi: 10.1186/s12896-017-0339-4

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© The Author(s). 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — Synthetic mTG construct used for transformation of E. coli BL21 (DE3) cells. The construct consists of restriction enzyme sites, the pro-domain required for folding and the mTG gene separately expressed. Each one has a preceding PelB sequence to direct periplasm secretion, whereupon folding will occur in the periplasm. Another ribosome binding site (RBS) precedes the mTG gene to ensure proper translation