Table 1.

Kinetic constants of WT and S2P variant measured by the mTG-GDH coupled enzyme method^a

Variant	Km (mM)	kcat (1/s)	kcat/Km (1/s⋅mM)
WT	11.6 ± 0.3	0.850 ± 0.007	0.073 ± 0.002
S2P	4.2 ± 0.1	0.60 ± 0.01	0.141 ± 0.002

^aResults are the average of three independent experiments with 5 assay repetitions in each experiment