Table 5.

Log₂-backtransformed least squares means of liver and polymorphonuclear leukocyte (PMNL) gene expression data in transition cows supplemented with rumen-protected methionine (MET) or unsupplemented (CON) at -10, +7, and +30 d from calving

						Diet × Time
Gene	Diet		Time			MET			CON			SEM1	P-value
	MET	CON	−10	+7	+30	−10	+7	+30	−10	+7	+30		Diet	Time	Diet × Time
Liver
GPR40	Not expressed
GPR84	0.77	0.84	0.88	0.74	0.80	0.85	0.73	0.75	0.91	0.75	0.86	0.14	0.64	0.25	0.89
GPR120	Not expressed
GPR109A	0.51	0.53	0.64	0.48	0.46	0.57	0.52	0.44	0.72	0.44	0.47	0.15	0.77	0.22	0.61
PMNL
GPR40	0.85*	1.13*	1.15a	0.95a,b	0.85b	1.00	0.80	0.77	1.33	1.14	0.94	0.17	0.04	0.04	0.78
GPR84	1.49*	0.65*	0.96	1.05	0.96	1.22	1.82	1.49	0.75	0.60	0.61	0.64	0.04	0.93	0.51
GPR120	Not expressed
GPR109A	1.34	0.87	1.26	0.91	1.10	1.42	1.31	1.28	1.11	0.63	0.94	0.44	0.09	0.60	0.71

^a,bStatistical difference (P < 0.05) among time points within the same group

*Statistical difference (P < 0.05) between groups within time points

¹Largest standard error of the mean