FIG 6.

Effect of enhanced expression of virB2 on efficiencies of T-DNA transfer from R. etli CFN42. N. benthamiana leaves were infiltrated with wild-type R. etli carrying p42a and R. etli carrying the pREp-virB2 construct, which expresses R. etli virB2 under the control of the R. etli virE promoter. Both bacterial strains also harbored the pCB302T-GFP reporter plasmid. The efficiency of T-DNA transfer was estimated by scoring the number of GFP-expressing cells per leaf surface area. All data represent average values from three independent experiments with the indicated standard deviations.