FIG 2.

Biofilm formation by wild-type TK1402 (TK1402), its isogenic alpB mutant strain (TK1402ΔalpB), and a TK1402 alpB restored strain (TK1402ΔalpB/alpB₁₄₀₂). (A) Quantification of H. pylori biofilms formed on glass coverslips after 3 days by CV staining and ethanol elution. The data are expressed as the means ± 1 standard deviation from at least three independent experiments. (B) CV-stained biofilm of wild-type TK1402 (TK1402), TK1402ΔalpB, and TK1402ΔalpB/alpB₁₄₀₂ strains of H. pylori grown on the surfaces of glass coverslips in brucella-FCS broth. An asterisk indicates significant difference (P < 0.05) relative to the level of wild-type biofilm biomass.