Western blot using antibody specific to rOmpA autotransporter. A Western blot of whole-cell rickettsial lysates probed with a specific antibody, c240, generated to the autotransporter region of rOmpA is shown. The peptide identified by Western blotting is specific only to strains carrying rOmpA and is the size predicted by cleavage of the autotransporter. Lanes: SS, Sheila Smith strain; KO, Sheila Smith ompA::int312 strain; Iowa, Iowa strain.