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The presence of rOmpA in the small-plaque Iowa strain was confirmed by mass spectrometry and immunofluorescence. (A) Coomassie brilliant blue-stained SDS-PAGE gel of rickettsial L and S Iowa variants. The high-molecular-weight bands were excised and identified by mass spectrometry. MW, molecular weight in thousands. (B) Immunofluorescence with a monoclonal antibody to rOmpA confirms the presence of the protein in the S but not the L variant. α, anti.
