TABLE 3.
Nonsynonymous SNPs distinguishing Iowa and confirming the large- and small-plaque variantsa
| Coordinate | Gene | SNP |
M_aa | Product | |||
|---|---|---|---|---|---|---|---|
| Iowa | Small-plaque variant | Large-plaque variant | Morgan | ||||
| 65860 | RrIowa_0091 | A | A | A | G | S260G | Succinate dehydrogenase iron-sulfur subunit |
| 194500 | RrIowa_0228 | A | A | A | G | K13E | Hypothetical protein |
| 229483 | RrIowa_0265 | A | A | A | C | L6W | Prolyl endopeptidase family |
| 250900 | RrIowa_0290 | A | A | A | G | *68W | Hypothetical protein/ankyrin |
| 857393 | RrIowa_1080 | A | A | A | G | M257T | Arp2/3 complex activation protein/RickA |
| 882319 | RrIowa_1113 | T | T | T | G | D27A | Hypothetical protein/ankyrin repeat |
| 1036868 | RrIowa_1321 | T | T | T | C | K99E | RNase H |
| 1090471 | RrIowa_1396 | A | A | A | G | I4M | Hypothetical protein/endonuclease subunit |
| 1127496 | RrIowa_1431 | A | A | A | G | K208R | Methyltransferase |
| 1252300 | RrIowa_1582 | A | A | A | G | F25S | Type I restriction-modification system methylation subunit |
a
Single-nucleotide polymorphisms (SNPs) which distinguish the R. rickettsii Iowa strain from Sheila Smith, R, and Morgan strains (34). Coordinates are from the reference Iowa strain (CP000766.3). M_aa, amino acid change in Morgan relative to the Iowa strain. The asterisk indicates a stop codon.