Table 3.
The relative expression levels of the particular genes in the parental strains and cat8Δ mutants at the third day of xylose alcoholic fermentation at 45 °C
| ΔΔCt | Genes | ||||||||||
|---|---|---|---|---|---|---|---|---|---|---|---|
| XYL1 | XYL2 | XYL3 | PDC1 | TKL1 | DAS1 | TAL1 | TAL2 | RPE1 | FBP1 | PCK1 | |
| cat8Δ/WT | 1.13 ± 0.300 | 1.05 ± 0.577 | 2.82 ± 0.438 | 0.64 ± 0.400 | 1.10 ± 0.361 | 2.39 ± 0.342 | 1.23 ± 0.360 | 1.21 ± 0.193 | 2.60 ± 0.486 | 0.67 ± 0.165 | 1.10 ± 0.435 |
| BEP cat8Δ/BEP | 0.36 ± 0.300 | 0.62 ± 0.085 | 1.07 ± 0.086 | 0.88 ± 0.479 | 0.79 ± 0.175 | 0.57 ± 0.195 | 1.11 ± 0.091 | 1.12 ± 0.067 | 1.54 ± 0.052 | 0.74 ± 0.392 | 0.76 ± 0.140 |
| BEP/WT | 7.66 ± 0.971 | 18.03 ± 0.045 | 2.76 ± 0.158 | 1.96 ± 0.380 | 1.71 ± 0.178 | 1.70 ± 0.670 | 9.29 ± 0.138 | 3.59 ± 0.138 | 47.60 ± 0.301 | 1.02 ± 0.274 | 0.19 ± 0.414 |
The mRNA quantification was normalized to ACT1 mRNA
Genes encode: XYL1, xylose reductase; XYL2, xylitol dehydrogenase; XYL3, xylulokinase; PDC1, pyruvate decarboxylase; TKL1, transketolase; DAS1, dihydroxyacetone phosphate synthase or peroxisomal transketolase; TAL1, transaldolase; TAL2, peroxisomal transaldolase; RPE1, ribulosephosphate epimerase; FBP1, fructose-1,6-bisphosphatase; PCK1, phosphoenolpyruvate carboxykinase