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Transition midpoint temperatures (T m) measured with the ThermoFAD method and the intrinsic tryptophan fluorescence
| Enzyme | ThermoFAD | Trp unfolding |
|---|---|---|
| E. coli (DH) | 57.7 ± 0.3 | 56.9 ± 0.4 |
| P. pastoris (CDH) | 59.2 ± 0.1 | 57.4 ± 0.6 |
| A. niger (CDH) | 59.7 ± 0.1 | 57.5 ± 0.5 |
| T. reesei (CDH) | 57.8 ± 0.2 | 56.6 ± 0.3 |
The measurements were done in triplicates
