Table 1.
Primer list- Real Time PCR
| Markers | Forward primer | Reverse primer | Tm | Product length |
|---|---|---|---|---|
| β-Actin | CATGTACGTTGCTATCCAGGC | CTCCTTAATGTCACGCACGAT | 60 | 250bp |
| VEGF-A | GCAGAATCATCACGAAGTGG | GCATGGTGATGTTGGACTCC | 60 | 234bp |
| TGF-β1 | CAGATCCTGTCCAAGCTG | TCGGAGCTCTGATGGTGTT | 56 | 270bp |
| Ang-1 | GCTTACCAGATTCACACTGTTCC | TTGCTACCTTGCCAACAACAACTG | 60 | 612bp |
| IL-6 | CACAGACAGCCACTCACCTC | TTTTCTGCCAGTGCCTCTTT | 60 | 137bp |
| HGF | ATGCATCCAAGGTCAAGGAG | TTCCATGTTCTTGTCCCACA | 61 | 349bp |
| SDF-1α/CXCL12 | TGCCAGAGCCAACGTCAAG | CAGCCGGGCTACAATCTGAA | 60 | 73bp |
| VEGFR1 | CGTAGAGATGTACAGTGAAA | GGTGTGCTTATTTGGACATC | 55 | 306bp |
| VEGFR2 | TTACAGATCTCCATTTATTGC | TTCATCTCACTCCCAGACT | 60 | 498bp |
| VEGFR3 | CTGGACCGAGTTTGTGGAGG | GTCACATAGAAGTAGATGAGCCG | 60 | 138bp |
Tm melting temperature, VEGF-A vascular endothelial growth factor A, TGF-β transforming growth factor beta, Ang-1 angiopoietin-1, IL interleukin, HGF hepatocyte growth factor, SDF-1 stromal-derived factor-1, VEGFR1 vascular endothelial growth factor receptor 1, VEGFR2 vascular endothelial growth factor receptor 2, VEGFR3 vascular endothelial growth factor receptor 3