Skip to main content
. 2017 Jan 17;36(5):664–678. doi: 10.15252/embj.201694831

Figure 6. The Rtt107 scaffold tethers DDK and Cdc5 to Mus81‐Mms4 independently of Slx4 and Dpb11.

Figure 6

  1. Rtt107, but not Slx4, is required for DDK and Cdc5 interaction with Mus81‐Mms4. Mms43FLAG pull downs from mitotically arrested cells as in Fig 1A, but specifically comparing interactions of Mus81‐Mms4 in WT, slx4Δ, rtt107Δ and slx4Δ rtt107Δ mutant backgrounds.
  2. Rtt107 interacts with Cdc7. Two‐hybrid interaction was tested using Gal4‐BD‐Rtt107 constructs and Gal4‐AD‐Cdc7 or Gal4‐AD‐Dbf4 constructs. Interaction between Gal4‐BD‐Cdc5 and Gal4‐AD‐Dbf4 serves as positive control.
  3. Rtt107 interacts with Mus81‐Mms4, DDK and Cdc5 independently of Slx4. Rtt1073FLAG co‐IPs from untagged control, WT or slx4Δ cells arrested in mitosis were probed for indicated proteins.
  4. Rtt107 interacts with Mus81‐Mms4 independently of the Mms4‐Dpb11 interaction. SILAC‐based Mms43FLAG pull down in WT and mms4‐S201A cells reveals changes in the Dpb11 association, but not in Rtt107, Slx4, Cdc5 or DDK binding. Plotted are the H/L ratios of two experiments including label switch.