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. 2016 Dec 21;36(5):583–603. doi: 10.15252/embj.201694591

Figure EV2. Amyloid plaque clearance is enhanced in the co‐culture of old APPPS1 and young WT brain slices.

Figure EV2

  • A
    Immunofluorescence analysis of core‐only plaques in the old APPPS1 slice in co‐culture (14 DIV) immunostained with mouse (M3.2, green) and human (2D8, red) Aβ antibodies reveals co‐localization due to co‐deposition of murine and human Aβ. Scale bar: 50 μm.
  • B, C
    Immunofluorescence analysis of old APPPS1 slices cultured alone or in co‐culture at 7 (B) and 14 (C) DIV and immunostained using M3.2. Amyloid plaque clearance is accelerated when old brain slices are co‐cultured together with young slices. Scale bar: 100 μm.
  • D
    Quantitative analysis of core‐only plaques in old APPPS1 brain slices cultured alone (old APPPS1 alone) or co‐cultured with young WT (old APPPS1 + young WT), young APPPS1 (old APPPS1 + young APPPS1), or old WT brain slices (old APPPS1 + old WT) at 14 DIV. Significantly enhanced amyloid plaque clearance is only observed upon co‐culturing of old APPPS1 brain slices with young slices (either WT or APPPS1). The values are expressed as percentages of core‐only plaques from the total number of amyloid plaques and represent mean ± SEM from three independent experiments, each experiment including three independent slice culture dishes (n.s. = not significant, ***P < 0.001; unpaired two‐tailed Student's t‐test).