Skip to main content
. 2017 Jan 25;36(5):629–645. doi: 10.15252/embj.201695803

Figure 5. Glycoform and C‐terminal clasp regulate α5β1 ectodomain conformational equilibria and have little effect on intrinsic affinities.

Figure 5

  • A
    SDS 7.5% PAGE of three α5β1 ectodomain glycoforms with or without C‐terminal clasp stained with Coomassie blue.
  • B, C
    Tabulation of results (B) and energy landscape plots (C). See Appendix Fig S4 for representative FP results. Error definitions and explanations of values marked with “*” and “#” are as in the Fig 4 legend.
  • D, E
    Effect of complex (D) and shaved (E) N‐glycans on clasped α5β1 ectodomain conformation in EM. Representative, well‐resolved class averages are shown together with their rank among 35 class averages and the % of total particles in that class (inset, upper left and right, respectively). Below each en face class average are the best cross‐correlating projections of open and closed headpiece crystal structures to the headpiece portion of the EM class average. Insets show correlation coefficients with the best correlating conformation in yellow. Scale bars are 10 nm. All class averages are shown in Appendix Fig S6.