FIG 10.

Modulation of tetherin, CD4, MHC-I, and CD74 by RBF206 Vpu and Nef proteins in their original HIV-1 O context. CD4⁺ T cells activated with CD3/CD28 beads were transduced with the infectious molecular clone RBF206 or the indicated nef- and/or vpu-defective mutants thereof and examined for tetherin, CD4, MHC-I, and CD74 surface expression 3 days later. The left side shows examples of primary data. Bar graphs show average levels (±SEM) of surface expression in virally infected (eGFP⁺) cells relative to uninfected cells. The mean fluorescence intensities (MFIs) used to calculate receptor downmodulation are indicated in the primary data. The sample infected with a vpu- and nef-defective construct represents 100%. Values were derived from independent infections of three donors. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.