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. 2017 Feb 28;91(6):e02177-16. doi: 10.1128/JVI.02177-16

FIG 8.

FIG 8

Modulation of surface molecules by RBF206 Vpu and Nef proteins in the context of NL4-3. (A) PHA-activated PBMCs were transduced with VSV-G-pseudotyped nef-defective HIV-1 NL4-3 UEOel IRES-eGFP constructs containing the indicated vpu alleles and examined for eGFP and tetherin or CD4 surface expression by flow cytometry 3 days later. The left side provides examples for primary data, and the right side shows average levels (±SEM) of surface expression in virally infected (eGFP+) cells relative to uninfected cells. The sample infected with a vpu-defective construct represents 100%. Values were derived from three independent infections of three donors. (B) PHA-stimulated PBMCs were transduced with VSV-G-pseudotyped NL4-3 IRES-eGFP constructs expressing the RBF206 Vpu and/or Nef proteins and analyzed by flow cytometry as described for panel A. Samples were additionally examined for MHC-I and CD74 surface expression. *, P < 0.05; **, P < 0.01; ***, P < 0.001; ****, P < 0.0001.