TABLE 1.
Description of antibodies used in the studya
| Target | Source | Vendor | Catalog no. |
|---|---|---|---|
| Actin | Rabbit | Sigma (St. Louis, MO) | A2066 |
| EGFR | Rabbit | Fitzgerald (Concord, MA) | 20R-ER004 |
| FLAG | Mouse | Sigma | F3165 |
| GFP | Mouse | Stressgen (San Diego, CA) | SAB-500 |
| HRS | Rabbit | Bethyl (Montgomery, TX) | A300-989A |
| Lamin B1 | Rabbit | Abcam (Cambridge, MA) | ab16048 |
| LAMP1 | Mouse | DSHBb | H4A3 |
| LAMP2 | Mouse | DSHB | H4B |
| NF-κB (p65) | Rabbit | Cell Signaling (Beverly, MA) | 8242 |
| NF-κB (pSer536) | Rabbit | Cell Signaling | 3033 |
| ORP1L | Mouse | Santa Cruz (San Diego, CA) | sc-376602 |
| ORP1L | Rabbit | Abcam | ab203352 |
| ORP5 | Rabbit | Novus (Littleton, CO) | NBP2-19683 |
| Rab5 | Mouse | BD Biosciences (San Jose, CA) | 610281 |
| Rab7 | Rabbit | Santa Cruz | sc6563 |
| SREBP1 | Mouse | Novus | NB600-582 |
| TLR4 | Mouse | R&D Systems (Minneapolis, MN) | MAB14782 |
| TfR | Mouse | Zymed (South San Francisco, CA) | 13-6800 |
| Tubulin | Rabbit | Cell Signaling | 2125 |
| VAP-A | Rabbit | Sigma | HPA009174 |
| VAP-B | Mouse | Proteintech (Rosemont, IL) | 14477-1-AP |
a
Antibodies were routinely used at a dilution of 1:1,000 to 1:2,000 for immunoblotting and at a dilution of 1:100 to 1:500 for immunostaining.
b
DSHB, Developmental Studies Hybridoma Bank (developed under the auspices of the Eunice Kennedy Shriver National Institute of Child Health and Human Development [NICHD] and maintained by The University of Iowa Department of Biological Sciences [Iowa City, IA]).