FIG 2.

Representative CyTOF scatter plots. The effective CD8⁺ T-cell clone EC1 and ineffective CD8⁺ T-cell clone IC2 were incubated with either unloaded HLA-B*2705-expressing GXR cells, KK10 peptide-loaded HLA-B*2705-expressing GXR cells or PMA and ionomycin (Iono) for 3 h. The cells were then stained with the indicated metal-tagged antibody specific for IFN-γ and CD107 (upper panels), CD40L and GM-CSF (middle panels), and IL-2 and TNF-α (lower panels) and analyzed by mass cytometry. The data are presented as dot plots.