TABLE 2.
Specific antibodies used for CyTOF staining and analysis
| Antibody | Label atomic mass | Ab clone; source |
|---|---|---|
| CD3 | QDot (112, 114, etc.) | S1.4; Invitrogen Qdot655 |
| IL-8 | 139 | E8N1; Biolegend |
| CD45RA | 141 | HI100; eBioscience |
| CD69 | 142 | MCA1442; AbD Serotec |
| CD5 | 143 | UCHT2; Biolegend |
| CD45RO | 144 | UCHL1; Biolegend |
| CD57 | 145 | HCD57; Biolegend |
| CD8 | 146 | HIT8a; Biolegend |
| GM-CSF | 147 | BVD2-21C11; Biolegend |
| CD11a | 148 | HI111; Biolegend |
| CD4 | 149 | SK3; Biolegend |
| MIP-1β | 150 | D21-1351; BD custom order |
| Granzyme B | 151 | 2CF/F5; BD |
| TNF-α | 152 | MAb11; eBioscience |
| CD107a/b | 153 | H4A3, H4B4; BD |
| CD27 | 154 | LG.7F9; eBioscience |
| PD1 | 155 | EH12.2H7; Biolegend |
| CD13 | 156 | WM15; Biolegend |
| CD19 | 157 | HiB19; Biolegend |
| KLRG1 | 158 | 13F12F2; kind gift from Hanspeter Pircher |
| CD56 | 159 | HiB19; Biolegend |
| CD28 | 160 | CD28.2; BD |
| CD38 | 161 | HIT2; eBioscience |
| IL-4 | 162 | MP4-25D2 |
| cCAS3 | 163 | C92-605; BD |
| IL-17 | 164 | BL168; Biolegend |
| CD40L | 165 | 24-31; Biolegend |
| IL-2 | 166 | MQ1-17h12; eBioscience |
| Integrin B7 | 167 | FIB504; Biolegend |
| CCR7 | 168 | 150503; R&D |
| MIP-1α | 176 | 11A3; BD custom order |
| IFN-γ | 170 | 4S.B4; eBioscience |
| HLA-DR | 171 | L243; BD |
| CD49d | 172 | 9F10; Biolegend |
| CTLA-4 | 173 | BN13; Biolegend |
| CD62L | 174 | DREG-56; BD |
| Perforin | 175 | B-D48; AbCam |
| Granzyme A | 176 | CB9; Biolegend |