TABLE 1.
Putative tail fibers/spikes/capsule depolymerases of phage K64-1a
| ORF | Type (location [nt]) | Product size (aa) | Homologue | Accession no | Sequence identity (%)b | Activityc |
|---|---|---|---|---|---|---|
| S1-1 | Complementary (325887–327995) | 702 | Tail fiber of Klebsiella phage K11 | YP_002003830.1 | 360/590 (61) | K11* |
| S1-2 | Complementary (321593–323803) | 736 | Tail spike protein head-binding protein of Klebsiella phage 0507-KN2-1 | YP_008532048.1 | 44/113 (39) | KN4† |
| S1-3 | Complementary (323855–325810) | 651 | Tail spike protein of Salmonella phage FSL SP-063 | AGF88658.1 | 44/140 (31) | K21* |
| S2-1 | Complementary (328067–331648) | 1193 | Putative tail fiber protein of Pectobacterium phage PP1 | YP_007010682.1 | 40/133 (30) | KN5* |
| S2-2 | Complementary (331729–333483) | 584 | Phage T7 tail fiber protein of Klebsiella pneumoniae | WP_020326882.1 | 177/496 (36) | K25† |
| S2-3 | Complementary (333493–335832) | 779 | K5 lyase of Enterobacter phage K1-5 | YP_654147.1 | 72/232 (31) | K35† |
| S2-4 | Complementary (335902–338568) | 888 | Tail fiber protein of Enterobacter phage EcP1 | YP_007003187.1 | 57/221 (26) | K1* |
| S2-5 | 338917–341907 | 996 | Putative tail fiber of Enterobacter phage RTP | YP_398994.1 | 171/579 (30) | K64* |
| S2-6 | 341980–344283 | 767 | Pectate lyase superfamily protein of Klebsiella pneumoniae | WP_020801644.1 | 161/462 (35) | K30, K69† |
| S2-7 | 344312–346471 | 719 | Tail fiber domain protein of Pseudomonas savastanoi pv. savastanoi NCPPB 3335 | EFH99537.1 | 45/96 (47) | |
| S2-8 | 90154–91941 | 595 | Putative tail fiber protein of Cronobacter phage vB_CsaM_GAP32 | YP_006987359.1 | 255/510 (50) |
a
nt, nucleotide; aa, amino acids.
b
As determined by BLAST-P.
c
*, Evidenced by protein expression; †, evidenced by both protein expression and phage mutants.