TABLE 5.
Primers used in this study
| Primer | Sequence (5′–3′) | Purpose |
|---|---|---|
| S1-1-Nhe1-Sac1-F | TGGGCTAGCGAGCTCGCAAATAAATTAACACAGCCAAAAGG | S1-1 expression |
| S1-1-Xho1-R(stop) | GGCTTTATTCTCGAGTTATCCAGCTAATATAAAAGAAACC | S1-1 expression |
| S1-2-NheI-PF | ATATGAGGTTAAGGCTAGCACAAATAGTTTAATACAACC | S1-2 expression |
| S1-2-XhoI-PR | ATATGGAGGCTCTCGAGTTAGCTATTGAATGATATTAC | S1-2 expression |
| S1-3-NheI-PF | TATATTTGGAGAAGCTAGCTAATGTCTACTGAATTAACAC | S1-3 expression |
| S1-3-XhoI-PR | TCATCAAAACTCGAGTTATATTAAAAATAGTCTAATATAAC | S1-3 expression |
| S2-1-Nhe1-Sac1-F | AGGGCTAGCGAGCTCGCATTTAAATTTAAAGGCTCACTATC | S2-1 expression |
| S2-1-Xho1-R(stop) | GGGGCTTTTTCTCGAGTTAACCAGACACTTGAATATTAAATG | S2-1 expression |
| S2-2-Nde1-F | TAGGATTAACATATGGGAAATTTTATACAACCTAAAG | S2-2 expression |
| S2-2-Xho1-R(stop) | GCTTTATTTTTCTCGAGTTATGCACCTCTAATATAAG | S2-2 expression |
| S2-3-Nhe1-Sac1-F | GAGGCTAGCGAGCTCATAAACGGATTAATTCAACCAAAAGGC | S2-3 expression |
| S2-3-Xho1-R(stop) | TCCCATATTCTCGAGCTATTTTTGTAATTGTTTTTC | S2-3 expression |
| S2-4-NheI-PF | ACAGCAAATTAAGCTAGCAAATGGAAACAGAGGGTTTAAC | S2-4 expression |
| S2-4-XhoI-PR | AGGAGGCTTCTCGAGTTATAATGATATTTGCCAATATATAG | S2-4 expression |
| S2-5-NheI-PF | TGCAAACTAAGAGGCTAGCACATGTCTTTAAGTAATTTAAG | S2-5 expression |
| S2-5-XhoI-PR | ACCCGAAGGTGCTTCTCGAGTTACTGTAAATAAATTCCTG | S2-5 expression |
| S2-6-Nhe1-Sac1-F | GAGGCTAGCGAGCTCTCATTAATTCAACTTTCACCAAGTAATG | S2-6 expression |
| S2-6-Xho1-R(stop) | CCTATTTATTTCTCGAGTTACCAAGTATTTATAGATAC | S2-6 expression |
| S2-7-Nde1-F | GATTTATCACATATGTCATTAACTAATTTAAACTC | S2-7 expression |
| S2-7-Xho1-R(stop) | GGCTTTTTTCTCGAGTTATATAGTTAAGAAACTTAC | S2-7 expression |
| S2-8-Nhe1-Sac1-F | AGGGCTAGCGAGCTCAGTATTAGTTTTAATCACCCCCAGAATAC | S2-8 expression |
| S2-8-XhoI-PR | TTATTTTCGTTCAACTCGAGTTATCGACCGATTGCTTGCC | S2-8 expression |
| S1-2 201F1 | GGCATATTACGTATGCGTTC | S1-2 mutant construct and check |
| S1-2 4434R2 | GTTTCTGGATATGCAGTTCG | S1-2 mutant construct and check |
| S1-2 gF | GGTTTTACACATTTCACAACTG | S1-2 mutant check |
| S1-2+48 inverse F | AAAACAAATTTTATCCGGCG | S1-2 mutant check |
| S1-2 A2 | TGGTGGTCAAATACATAGAC | S1-2 mutant check |
| S1-2 PR | TTAGCTATTGAATGATATTAC | S1-2 mutant check |
| S2-2+1034F | TGCTTATTCAAGTACTGACG | S2-2 mutant construct and check |
| S2-2+1007R | CTATCCAAGAAAACTAATACTTC | S2-2 mutant construct and check |
| S2-2-125IF | GTACAAATACACCTACTGGG | S2-2 mutant construct |
| S2-2-35IR | GAAGTTGAACCTTTAGGTTG | S2-2 mutant construct |
| S2-2_g+1061F | GCCGCCAAGTAACAACGAAT | S2-2 mutant check |
| S2-2_g+1208R | ATCACCTTCTTGTAGTGGTG | S2-2 mutant check |
| 11_S2_ORF2F PF | ATATGGGAAATTTTATACAAC | S2-2 mutant check |
| 11_S2_ORF2R PR | GCACCTCTAATATAAGCTTG | S2-2 mutant check |
| S2-3+1079F | TCAGGGGTGGTATGGTGTAG | S2-3 mutant construct and check |
| S2-3+1005R | CCGTTACGGTCAATCAATTCCC | S2-3 mutant construct and check |
| S2-3_inverseF | TAGGATTAAAATATGGGAAATTTTATAC | S2-3 mutant construct |
| S2-3_inverseR | TACATTAACCTCAATTTACAATATAC | S2-3 mutant construct |
| S2-3_g+1159F | CACAGGTGATGTTAGCGGAA | S2-3 mutant check |
| S2-3_g+1087R | GAGGATATTCTTCCGTTTCG | S2-3 mutant check |
| 11_S2_ORF3F PF | TAAATGATAAACGGATTAATTC | S2-3 mutant check |
| 11_S2_ORF3R PR | TTTTGTAATTGTTTTTCAATTTC | S2-3 mutant check |
| S2-6+793F | TTGTTCATTTGTGGGGTTAG | S2-6 mutant construct and check |
| S2-6+965R | TACATTATCACCTGCTGGCC | S2-6 mutant construct and check |
| S2-6 inverseF | CACACTGAGAACCAAATATG | S2-6 mutant construct |
| S2-6 inverseR | TTAATAAACCTCGTTATAAAG | S2-6 mutant construct |
| S2-6+1068gF | TCGAACCGTGGGGATTTAAAG | S2-6 mutant check |
| S2-6+1050gR | ACCAAGTGTCAAACTACCAC | S2-6 mutant check |
| S2-6 5B | CAACTATATGTCCTCGACCA | S2-6 mutant check |
| S2-6 5A | GTATGGAGTGGTGTTGGTGT | S2-6 mutant check |