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. 2017 Feb 28;91(6):e02296-16. doi: 10.1128/JVI.02296-16

FIG 5.

FIG 5

Ultrasensitive p24 analysis reveals differences among LRA in reactivating HIV from resting CD4+ T cells. Samples from the study of Noel et al. (20) were analyzed here with the ultrasensitive p24 assay. Resting CD4+ T cells from 10 HIV-infected individuals with undetectable viremia (cART-treated and HIV controllers) were stimulated by different latency-reversing agents (prostratin, chaetocin, SAHA, 5-AzadC [5′AZA], HMBA, and IL-7). Nonstimulated cells (mock) and anti-CD2 plus anti-CD28 (2/28), PHA plus IL-2 (PHA), and preactivated allogeneic CD8-depleted T cells (Allo) were used as controls. Cultures were monitored at days 1, 3, 6, and 14 for HIV-1 RNA production. (A) Kinetics of viral reactivation/stimulus measured by HIV-RNA is shown. (B) Relationship between the amount of p24 and HIV-1 RNA in the culture supernatants from resting CD4+ T cells stimulated by different LRA. Correlation was calculated using a nonparametric Spearman test. Samples positive for p24 are represented in orange, and samples negative for p24 are represented in blue. (C) RNA levels for all the supernatants analyzed/stimulus are indicated. Samples positive for p24 are represented by orange circles, and samples negative for p24 are represented by blue circles. Pie charts represent the frequency of positive p24 samples (orange) per stimulus.