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. 2017 Feb 28;91(6):e01857-16. doi: 10.1128/JVI.01857-16

FIG 4.

FIG 4

Mapping of the NAb 13B5 minimal binding site. (A and B) C- and N-terminally truncated peptides based on library peptide 40 were used in an ELISA to identify the shortest amino acid sequence needed for binding of NAb 13B5. (C) ELISA to compare 13B5 binding to K13M, comprising the minimal 13B5 epitope sequence, and peptides based on K13M with one (H14M) or two (K15M) additional amino acid residues of UL128 added to the N terminus. (D) Alanine scanning based on peptide K13M to identify amino acid residues involved in 13B5 binding. Bars represent standard deviations of data from triplicate wells. Vertical axes represent the optical density at 450 nm.