FIG 1.

Hsp90 activity is required for MuV propagation. (A) Schematic of the rOdate and rOdate/AcGFP genes. SH, small hydrophobic gene. (B) Vero cells infected with rOdate or rOdate/AcGFP were observed under phase-contrast and a fluorescence microscopes at 48 hpi. (C) Vero cells were infected with rOdate or rOdate/AcGFP at an MOI of 0.01. The supernatants were collected at 24, 48, 72, and 96 hpi, and the infectious titers were determined by plaque assay. (D) Vero cells were treated with the indicated concentrations of 17-AAG for 96 h, and then cell viability was determined and calculated as a percentage of the viability of cells treated with DMSO. (E and F) Vero cells were infected with rOdate/AcGFP at an MOI of 0.01 and treated with the indicated concentrations of 17-AAG. At 96 hpi, the cells were observed under a fluorescence microscope (E), and the infectious titers in the supernatants were determined (F). (G) A549 cells were treated with 0.1 and 0.2 μM 17-AAG for 96 h, and then cell viability was determined and calculated as a percentage of the viability of cells treated with DMSO. (H and I) A549 cells were infected with rOdate/AcGFP at an MOI of 0.01 and treated with 0.1 μM 17-AAG. At 96 hpi, the cells were observed under a fluorescence microscope (H), and the infectious titers in the supernatants were determined (I). Error bars indicate the standard deviations of triplicates. The significance of differences between the means was determined by Student's t test. *, P < 0.05; **, P < 0.01. ND, not detected; DAPI, 4′,6′-diamidino-2-phenylindole.