FIG 9.

Hsp70 and CHIP are also associated with measles virus (MeV) L protein and involved in MeV infection. (A) 293T cells were transfected with pCAGGS-HA-MeV-L, and the medium was replaced with fresh medium containing 0.5 μM 17-AAG at 12 h posttransfection. At 24 h posttransfection, the cell lysates were subjected to immunoblotting with anti-HA and anti-β-actin antibodies. (B) 293T cells were transfected with pcDNA-CHIP-Myc/His (WT) and/or pCAGGS-HA-MeV-L. At 24 h posttransfection, the cell lysates were subjected to immunoprecipitation with anti-HA antibody and to immunoblotting with the indicated antibodies. (C) Vero/hSLAM cells were infected with IC323-EGFP at an MOI of 0.01 and treated with the indicated concentrations of VER155008 with/without 0.2 μM 17-AAG. At 72 hpi, the infectious titers in the supernatants were determined. Error bars indicate the standard deviations of triplicates. ND, not detected.