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M21T significantly enhanced viral assembly efficiency. Full-length JFH1 RNAs containing WT or M21T NS3 were electroporated into Huh7.5.1 cells, and the cells were harvested at day 2 and day 3 postelectroporation. (A) Intracellular HCV RNA levels were analyzed by quantitative reverse transcription-PCR (RT-qPCR). Extracellular (B) and intracellular (C) infectivities were determined by titration assay. Error bars indicate standard deviations calculated from 3 independent experiments.
