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Immunostaining for chimeric VSVs in mouse brain cultures containing neurons and glia. (A and B) VSVΔG-Nipah F+G and VSVΔG-H5N1 showed immunostaining in small neurons (arrows). Scale bar, 30 μm (all panels). (C) VSVΔG-CHIKV showed immunoreactivity in the plasma membranes of large astrocytes (long arrows) and also staining in some small neuron-like cells (short arrows). (D) VLV showed staining in neurons (arrows). (E) Control cultures with no virus showed no immunoreactivity.
