FIG 6.

VSVΔG-CHIKV in brain and culture. (A) Injection of VSVΔG-CHIKV into the brain infected both neurons and astrocytes at 3 dpi. The arrow points to an astrocyte with many radiating processes. Bar, 35 μm. (B) No infection was seen in the contralateral cortex. Bar, 25 μm. (C) Some neurons also showed VSVΔG-CHIKV infection. Bar, 25 μm. (D) In culture, large, flat astrocytes are preferentially infected at 1 dpi. (E) Astrocytes are infected in vitro at 1 dpi. Bar, 12 μm. (F) The arrows indicate cells with neuronal morphology in the same field shown in panel E. Whereas many glial cells were infected, neurons were not infected. Bar, 30 μm.