TABLE 5.

Primers and DNA template used in this study

Primer name	Sequence (5′ to 3′)a
PB1-for	ACGACCGGTACCGCCACCACCATGGATGTCAATCCGACCTTACTTTTC
PB1-rev	ATGCATGCGCGGCCGCCTATTTTTGCCGTCTGAGCTCTTCAAT
V43I-fof	CACCATGGATACTATCAACAGGACACATC
V43I-rev	GATGTGTCCTGTTGATAGTATCCATGGTG
V43A-for	CACCATGGATACTGCCAACAGGACACATC
V43A-rev	GATGTGTCCTGTTGGCAGTATCCATGGTG
V43L-for	CACCATGGATACTCTCAACAGGACACATC
V43L-rev	GATGTGTCCTGTTGAGAGTATCCATGGTG
V43 M-for	CACCATGGATACTATGAACAGGACACATC
V43 M-rev	GATGTGTCCTGTTCATAGTATCCATGGTG
V43S-for	CACCATGGATACTAGCAACAGGACACATC
V43S-rev	GATGTGTCCTGTTGCTAGTATCCATGGTG
V43T-for	CACCATGGATACTACCAACAGGACACATC
V43T-rev	GATGTGTCCTGTTGGTAGTATCCATGGTG
Pol1-for	GTGTGTCCTGGGGTTGACCAGA
Pol1-rev	CATCGGTGATGTCGGCGATATAG
Bm-Uni-for	CGTATTCGTCTCAGGGAGCRAAAGCAGG
Bm-Uni-rev	CGATATCGTCTCGTATTAGTAGAAACAAGG
pdm-seg4-for	AGCAAAAGCAGGGGAAAATAAAAGC
pdm seg4-rev	AGTAGAAACAAGGGTGTTTT
pdm-seg6-for	AGCAAAAGCAGGAGTTTAAAATG
pdm-seg6-rev	AGTAGAAACAAGGAGTTTTTT
Seg8-for	CGTATCGCCTCCCTCGCGCCATCAGAGCACTGTAGTGTGTCAAGCTTTCAGGTAG
Seg8-rev	CTATGCGCCTTGCCAGCCCGCTCAGAGCACTGTAGGTCCATTCTGATACAAAGAGG
T7 pro-120 mer DNA	TAATACGACTCACTATTAGTAGAAACAAGGTCGTTTTTCCTCTGTTCGTCTCTGCTTGTTGGCCTTCCGTGTTCTTACGTTCGCTTTCTTCGTTCTTTTGTTCTTTCCGTCTTGGTTGTCGTTGTCCTGCTTTCGCT

^aUnderlining indicates the codon sequence corresponding to amino acid 43 of the PB1 protein.