FIG 8.

Efficacy of melanin in vitro and in vivo. (A) Immunoblot data for 263K prion-derived PrPres after PMCA reaction in the presence of tyr-melanin. Representative data from duplicate experiments are shown. Anti-PrP monoclonal antibody 3F4 was used for PrP detection. (B) Kaplan-Meier graph of the albino and control animals. Both albino B10.C-Tyr<c>/Hir and control C57BL/10JMsHir mice were infected intracerebrally or intraperitoneally with 22L prion and observed until the disease became terminal. (C) Kaplan-Meier graph of animals treated with melanin. Female Tg7 mice intraperitoneally infected with 263K prion were given a single subcutaneous injection of tyr-melanin at a tolerance dose (50 mg) or vehicle 3 days postinfection and observed until the disease became terminal.