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. Author manuscript; available in PMC: 2017 Aug 1.
Published in final edited form as: Arthritis Rheumatol. 2016 Aug;68(8):1887–1898. doi: 10.1002/art.39618

Figure 5. SOD2 acetylation is inversely correlated with SIRT3 protein content in an age-dependent manner in human OA cartilage.

Figure 5

Acetylated SOD2 protein was detected in human OA cartilage by Western blot and compared to SIRT3 protein content as a function of age. (A) Representative SOD2 AceK68 and SOD2 Western blot images. SOD2 relative density was normalized to sample-matched β-ACTIN (r2=0.008, P = 0.80, n=10). SOD2 AceK68 normalized to SOD2 increased with age (r2=0.74, P = 0.0013, n=10). (B) Representative SIRT3 and β-ACTIN Western blot images. SIRT3 normalized to β-ACTIN showed a decreasing trend with age (r2=0.40, P = 0.066, n=9). (C) SIRT3-mediated deacetylation increased SOD2 activity in human OA cartilage (P = 0.0007, n=6).