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. 2017 Feb 20;10(1):e001598. doi: 10.1161/CIRCGENETICS.116.001598

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© 2016 The Authors.

Circulation: Cardiovascular Genetics is published on behalf of the American Heart Association, Inc., by Wolters Kluwer Health, Inc. This is an open access article under the terms of the Creative Commons Attribution Non-Commercial-NoDervis License, which permits use, distribution, and reproduction in any medium, provided that the original work is properly cited, the use is noncommercial, and no modifications or adaptations are made.

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Figure 4. — Histological analysis of Hyal2^−/− and control hearts at embryonic day (E) 18.5. Paraffin sections from E18.5 embryos were stained or used for immunohistochemistry. A and B, Images of hematoxylin and eosin–stained embryo sections revealed an enlarged atrium (open arrow) and the presence of fibrosis (arrow) in the Hyal2^−/− heart (A) compared with the control (B) heart. (Hyal2^−/−, n=8; control, n=6). C–F, Enlarged view of the area in the box in (A) and (B) stained for hyaluronan (HA). C and D, Images showing increased HA (brown) in the Hyal2^−/− atrium (C) compared with the control atrium (D; n=3). E and F, Detection of vimentin-positive cells (brown) revealed an excess in the Hyal2^−/− atrium (E) compared with the control (F; n=3). G–H, Semi-quantitative analysis of vimentin-positive cells in the atrium (G; n=3) and ventricle (H; n=4) of Hyal2^−/− and control hearts. There were significantly increased numbers of mesenchymal cells in the Hyal2^−/− hearts compared with controls. Bar=50 μm. LA, left atrium; LV, left ventricle; and RV; right ventricle. *P<0.05; **P<0.001.