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Matrix deposition by adipose-derived stem cells (ADSCs) on plasma modified scaffolds after 3 weeks of chondrogenic differentiation. A-H) Detection of aggrecan (A-D) and collagen type II (E-H) expression (both green) by immunocytochemistry. Negligible levels of expression are detected in undifferentiated controls (H). Nuclei are in blue (DAPI staining). Scale bar: 250 μm. I) Negative control where primary antibody was omitted.
